A new high-capacity cosmid vector and its use
Abstract
The construction of a cosmid, MUA-3, designed for the convenient cloning of eukaryotic DNA segments up to 48 kb in length is described. The cosmid contains all of the plasmid pBR322 with approx. 400 bases of λ DNA, including the cohesive end site, inserted at the pBR322 PstI endonuclease recognition site. Methods for using this vector to construct several types of Drosophila melanogaster genomic DNA libraries are given, and libraries made by these methods are characterized. A sheared Drosophila DNA-EcoRI linker library is shown to stably maintain average Drosophila DNA inserts of over 40 kb and up to 48 kb, and the efficiency of producing clones by a partial restriction and ligation method is shown to be over 3 × 10^5 clones/μg of Drosophila DNA.
Additional Information
© 1980 Elsevier. Received 13 April 1980, Accepted 25 June 1980. Communicated by J. Carbon. This work was supported by research grants from the National Science Foundation, the National Institute of Health and the American Cancer Society. E.M.M. and G.M.G. were fellows of the Jane Coffin Childs Memorial Fund for Medical Research, G.M.G. was also supported in part by the American Cancer Society - California Division.Additional details
- Eprint ID
- 72747
- Resolver ID
- CaltechAUTHORS:20161212-163941771
- NSF
- NIH
- American Cancer Society
- Jane Coffin Childs Memorial Fund for Medical Research
- American Cancer Society, California Division
- Created
-
2016-12-13Created from EPrint's datestamp field
- Updated
-
2021-11-11Created from EPrint's last_modified field