Optogenetically enhanced pituitary corticotroph cell activity post-stress onset causes rapid organizing effects on behaviour
Abstract
The anterior pituitary is the major link between nervous and hormonal systems, which allow the brain to generate adequate and flexible behaviour. Here, we address its role in mediating behavioural adjustments that aid in coping with acutely threatening environments. For this we combine optogenetic manipulation of pituitary corticotroph cells in larval zebrafish with newly developed assays for measuring goal-directed actions in very short timescales. Our results reveal modulatory actions of corticotroph cell activity on locomotion, avoidance behaviours and stimulus responsiveness directly after the onset of stress. Altogether, the findings uncover the significance of endocrine pituitary cells for rapidly optimizing behaviour in local antagonistic environments.
Additional Information
© 2016 The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. Received: 01 October 2015; Accepted: 18 July 2016; Published online: 20 September 2016. We thank L. Castillo-Ramírez and C.-M. Yeh for assistance with the experiments, A. Gutierrez-Triana for the mc2r probe, G. Shoeman, R. Singer and A. Schoell for expert fish care, K. Schmidt, R. Rödel, M. Lukat and N. Neef for technical support, and M. Gil and C. Lafourcade for suggestions. This work was supported by DFG-FOR1279, the Max Planck Society and Behrens-Weise Foundation. Author Contributions: R.J.D.M. and S.R. conceived the research. R.J.D.M. designed the experiments and oversaw the project. S.R. generated the transgenic line. R.J.D.M., T.T., A.H.G. and U.H. performed the experiments. R.J.D.M. analysed the data with assistance of T.T. and A.H.G. R.J.D.M. and S.R. wrote the paper with the assistance of T.T. and U.H. Competing interests: R.J.D.M. and S.R. are authors of the EP Patent application 13 80 2343.7, 'Novel inducible animal model of stress behavior.' The remaining authors declare no competing financial interests.Attached Files
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Additional details
- PMCID
- PMC5034294
- Eprint ID
- 71944
- Resolver ID
- CaltechAUTHORS:20161111-105657753
- DFG-FOR1279
- Deutsche Forschungsgemeinschaft (DFG)
- Max Planck Society
- Behrens-Weise Foundation
- Created
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2016-11-17Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field