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Published September 13, 2016 | Published
Journal Article Open

Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholera and are stabilized by a double signaling domain receptor DosM

Abstract

Nearly all motile bacterial cells use a highly sensitive and adaptable sensory system to detect changes in nutrient concentrations in the environment and guide their movements toward attractants and away from repellents. The best-studied bacterial chemoreceptor arrays are membrane-bound. Many motile bacteria contain one or more additional, sometimes purely cytoplasmic, chemoreceptor systems. Vibrio cholerae contains three chemotaxis clusters (I, II, and III). Here, using electron cryotomography, we explore V. cholerae's cytoplasmic chemoreceptor array and establish that it is formed by proteins from cluster I. We further identify a chemoreceptor with an unusual domain architecture, DosM, which is essential for formation of the cytoplasmic arrays. DosM contains two signaling domains and spans the two-layered cytoplasmic arrays. Finally, we present evidence suggesting that this type of receptor is important for the structural stability of the cytoplasmic array.

Additional Information

© 2016 National Academy of Sciences. Edited by Caroline S. Harwood, University of Washington, Seattle, WA, and approved July 19, 2016 (received for review March 22, 2016). Published online before print August 29, 2016. We thank Drs. Zhiheng Yu, Jason de la Cruz, Chuan Hong, and Rick Huang for microscopy support at the Howard Hughes Medical Institute Janelia Research Campus. This work was supported by National Institute of General Medical Sciences Grant GM101425 (to G.J.J.) and by the Max Planck Society (S.R.). Author contributions: A.B., D.R.O., S.R., and G.J.J. designed research; A.B., D.R.O., P.M., and A.K. performed research; A.B., D.R.O., P.M., A.K., and S.R. analyzed data; and A.B., D.R.O., S.R., and G.J.J. wrote the paper. The authors declare no conflict of interest. This article is a PNAS Direct Submission. Data deposition: The electron cryotomography subtomogram average density map reported in this paper has been deposited in the Electron Microscopy Data Bank, www.emdatabank.org (accession no. EMD-3398).

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August 22, 2023
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