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Published June 15, 2010 | Published
Journal Article Open

Calibration-free absolute quantification of optical absorption coefficients using acoustic spectra in 3D photoacoustic microscopy of biological tissue

Abstract

Optical absorption is closely associated with many physiological important parameters, such as the concentration and oxygen saturation of hemoglobin, and it can be used to quantify the concentrations of nonfluorescent molecules. We propose a method to use acoustic spectra of photoacoustic signals to quantify the absolute optical absorption. This method is self-calibrating and thus insensitive to variations in the optical fluence. Factors such as system bandwidth and acoustic attenuation can affect the quantification but can be canceled by dividing the acoustic spectra measured at two optical wavelengths. Using optical-resolution photoacoustic microscopy, we quantified the absolute optical absorption of black ink samples with various concentrations. We also quantified both the concentration and oxygen saturation of hemoglobin in a live mouse in absolute units.

Additional Information

© 2010 Optical Society of America. Received February 9, 2010; revised May 14, 2010; accepted May 9, 2010; posted June 1, 2010 (Doc. ID 123979); published June 10, 2010. This work was sponsored in part by National Institutes of Health (NIH) grants R01 EB000712, R01 EB008085, R01 CA113453901, U54 CA136398, and 5P60 DK02057933. L. W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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