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Published June 2015 | public
Book Section - Chapter

3D silicon electrodes with built-in glass capillaries for dielectrophoretic single-cell positioning and anaylisis

Abstract

We present microfluidic designs for dielectrophoretic positioning of single cells and their subsequent analysis based on 3D silicon electrodes featuring built-in capillaries. The designs are presented through numerical simulations as well as experimental validations showing effectiveness of dielectrophoretic forces in trapping single cells. Single-cell experiments are also demonstrated including viability assays and ionophore-mediated intracellular calcium flux assays. Seal resistance measurements between the capillaries and cells trapped, which are essential for patch-clamp assays, are reported.

Additional Information

© 2015 IEEE. Financial support was provided by the Research Grant Council of Hong Kong GRF 16211714.

Additional details

Created:
September 15, 2023
Modified:
October 23, 2023