Published June 5, 1976
| public
Journal Article
The in vitro transcription units of bacteriophage φX174. II. In vitro initiation sites of φX174 transcription
- Creators
- Smith, L. H.
- Sinsheimer, R. L.
Abstract
Employing the 5′ proximal labeling procedure reported previously, and hybridization to Haemophilus endonuclease bacteriophage φX RF DNA fragments, we have detected three major sites of initiation of in vitro φX transcripts. These three sites have been located on the φX174 genetic map in the following regions: (1) near the 5′ end of gene A, (2) near the 5′ end of gene B, and (3) near the 5′ end of gene D.
Additional Information
© 1976 Elsevier Ltd. Received 30 June 1975. and in revised form 15 December 1975. We are greatful to Dr R. Espejo for his gift of ^3H-labeled φX DNA and his suggestion to use it as an internal hybridization control. We also wish to thank Drs P.H. Johnson and A. S. Lee for their generous gift of Haemophilus endonuclease preparations. This research was supported in part by grant GM13554 from the National Institutes of Health. We thank the following for their comments and discussion: Drs. J. Strauss, J. Vinograd, W. Wood, H. Mitchell and H. Rohwer.Additional details
- Eprint ID
- 69427
- DOI
- 10.1016/0022-2836(76)90204-7
- Resolver ID
- CaltechAUTHORS:20160804-074258390
- GM 13554
- U.S. Public Health Service (USPHS)
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2016-08-04Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field