Published March 28, 1971
| public
Journal Article
Genetic information in Oocyte RNA
- Creators
- Davidson, Eric H.
- Hough, Barbara R.
Abstract
RNA of the mature Xenopus oocyte was hybridized with the isolated non-repetitive DNA fraction of the Xenopus genome. The hybrids had the thermal stability and other characteristics expected of non-repetitive DNA-RNA hybrids, and DNA recovered from them reassociated at the rate of non-repetitive DNA. By saturating the non-repetitive DNA with Oocyte RNA a direct minimum estimate was obtained of the amount of non-repetitive sequence in the oocyte RNA, i.e. its genomic information content. The oocyte RNA contains about 20 × 10^6 nucleotide pairs of diverse sequence, which is 4.5 times the total genomic information present in the Escherichia coli chromosome.
Additional Information
© 1971 Elsevier Ltd. Received 4 September 1970, Revised 20 November 1970. This research was supported by the American Cancer Society grant no. E334 and by the National Institute of Child Health and Development grant no. HD-02412. We are extremely grateful to Dr Jack Goldstein of the New York Blood Center for assisting us with the polyacrylamide gel determinations. We are deeply indebted to Dr Roy Britten, of the Carnegie Institution of Washington and to Dr Bruce R. Voeller of this University for many penetrating and helpful discussions and for critical advice on this manuscript. We take great pleasure in acknowledging the skill of Mr William Viets and also of Mr Harris Huberman in manually collecting oocytes, and the intelligent and professional assistance of Miss Maggie Chamberlin.Additional details
- Eprint ID
- 69396
- DOI
- 10.1016/0022-2836(71)90396-2
- Resolver ID
- CaltechAUTHORS:20160802-144344771
- E334C
- American Cancer Society
- HD-02412
- NIH
- Created
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2016-08-02Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field