The process of infection with bacteriophage φX174. XVIII. Intracellular Antiserum-precipitable Components
Abstract
Components precipitable by antiserum to bacteriophage φX174 can be derived from φX-infected cells. Antiserum-precipitable ^(35)S is found in three peaks after zone sedimentation in sucrose gradients. The fastest sedimenting peak is infective virus; the intermediate peak is the non-infective 70 s component. The slowest component was studied in greater detail and has the following properties. It has a sedimentation coefficient of about 6; in a thymine-requiring host, the material accumulates in the absence of thymidine; the accumulated material is subsequently incorporated into virus when thymidine is restored. The 6 s material is, therefore, considered to be a subunit of the virus structure. The φX double mutant am3tsγ, which does not make whole virus under restrictive conditions, forms a subunit which appears to have similar sedimentation properties to that of the wild type.
Additional Information
© 1968 Elsevier Ltd. Received 4 August 1967, and in revised form 6 November 1967. We wish to thank Dr Stanley Krane for helpful assistance during the initial stage of these experiments, and for making a generous supply of φX antiserum available. This research was supported in part by grants RG6965 and GM13554 from the U.S. Public Health Service. One of the authors (L.L.G.) was a post-doctoral research fellow from 1963 to 1965.Additional details
- Eprint ID
- 69395
- Resolver ID
- CaltechAUTHORS:20160802-142741728
- RG6965
- U.S. Public Health Service (USPHS)
- GM13554
- U.S. Public Health Service (USPHS)
- Created
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2016-08-02Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field