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Published July 1975 | public
Journal Article

Sequence complexity of heterogeneous nuclear RNA in sea urchin embryos

Abstract

The sequence complexity of heterogeneous nuclear RNA in sea urchin gastrulas was measured by RNA-driven hybridization reactions with nonrepetitive sea urchin DNA. 28.5% of the sequence complexity of the genome is represented in the nuclear RNA. This amounts to 1.74 × 10^8 nucleotides of diverse sequence, more than 10 times the nucleotide complexity of the polysomal messenger RNA extracted from sea urchin embryos at the same stage. The complex set of nuclear RNA sequences driving this hybridization reaction was shown to be the same as the rapidly labeled hnRNA, using pulse-labeled nuclear RNA as driver.

Additional Information

© 1975 MIT. Received March 25, 1975; revised April 24, 1975. We are pleased to acknowledge the competent technical assistance of Paul H. Yancey and Michael Kozlowski. This work was supported by grants from the National Institutes of Health and the National Science Foundation.

Additional details

Created:
August 19, 2023
Modified:
October 20, 2023