Published August 1974
| public
Journal Article
A Continuous Electroelution Method for the Recovery of DNA Restriction Enzyme Fragments
- Creators
- Lee, Amy Shiu
- Sinsheimer, Robert L.
Abstract
Bacterial restriction enzymes are deoxyribonucleases which cleave double-stranded DNA at specific sites and produce unique, high molecular-weight fragments. These enzymes provide a most powerful tool for studying the structure and function of DNA. The principal method for separation of the DNA fragments is electrophoresis through acrylamide or agarose gels. However, when fragments are to be isolated for further analysis, continuous elution from long cylindrical gels allows maximum separation and recovery.
Additional Information
© 1974 Elsevier Inc. Received 17 December 1973, Accepted 20 February 1974. The authors thank Dr. K. Grohmann and Dr. P. Johnson for helpful discussion. This work was supported by U. S. Public Health Service Grant GM 13554.Additional details
- Eprint ID
- 69285
- DOI
- 10.1016/0003-2697(74)90279-6
- Resolver ID
- CaltechAUTHORS:20160728-125514059
- GM 13554
- U.S. Public Health Service (USPHS)
- Created
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2016-07-28Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field