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Published June 24, 2016 | Accepted Version + Supplemental Material
Journal Article Open

Reprogramming of avian neural crest axial identity and cell fate

Abstract

Neural crest populations along the embryonic body axis of vertebrates differ in developmental potential and fate, so that only the cranial neural crest can contribute to the craniofacial skeleton in vivo. We explored the regulatory program that imbues the cranial crest with its specialized features. Using axial-level specific enhancers to isolate and perform genome-wide profiling of the cranial versus trunk neural crest in chick embryos, we identified and characterized regulatory relationships between a set of cranial-specific transcription factors. Introducing components of this circuit into neural crest cells of the trunk alters their identity and endows these cells with the ability to give rise to chondroblasts in vivo. Our results demonstrate that gene regulatory circuits that support the formation of particular neural crest derivatives may be used to reprogram specific neural crest–derived cell types.

Additional Information

© 2016 American Association for the Advancement of Science. Received 18 January 2016; accepted 23 May 2016. We thank J. Tan-Cabugao, M. Stone, B. Jun, and D. S. E. Koo for technical assistance. The Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory provided sequencing and bioinformatics support. We are indebted to D. Perez, K. Beadle, and R. Diamond for cell-sorting assistance at the Caltech Flow Cytometry Cell Sorting Facility. We also thank M. Barembaum for the Sox8 and Tfap2b expression constructs. This work was supported by NIH grants DE024157 and HD037105 to M.E.B. M.S.-C. was funded by a fellowship from the Pew Fellows Program in Biomedical Sciences and by NIH grant K99DE024232. The supplementary materials contain additional data.

Attached Files

Accepted Version - nihms825478.pdf

Supplemental Material - aaf2729-Simoes-Costa-SM.pdf

Supplemental Material - aaf2729-Simoes-Costa-SM.table.S1.xlsx

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