A Microfluidic Device for Kinetic Optimization of Protein Crystallization and In Situ Structure Determination
Abstract
The unprecedented economies of scale and unique mass transport properties of microfluidic devices made them viable nano-volume protein crystallization screening platforms. However, realizing the full potential of microfluidic crystallization requires complementary technologies for crystal optimization and harvesting. In this paper, we report a microfluidic device which provides a link between chip-based nanoliter volume crystallization screening and structure analysis through "kinetic optimization" of crystallization reactions and in situ structure determination. Kinetic optimization through systematic variation of reactor geometry and actuation of micromechanical valves is used to screen a large ensemble of kinetic trajectories that are not practical with conventional techniques. Using this device, we demonstrate control over crystal quality, reliable scale-up from nanoliter volume reactions, facile harvesting and cryoprotectant screening, and protein structure determination at atomic resolution from data collected in-chip.
Additional Information
© 2006 American Chemical Society. Received November 10, 2005. Publication Date (Web): February 15, 2006. This work was funded by National Institutes of Health (R01 HG003594, R01-CA77373), the Natural Sciences and Engineering Research Council of Canada (J. Payette Fellowship), the American Cancer Society (PF-03-124-01-GMC), the Mathers Foundation, and the National Cancer Institute (CA92584).Attached Files
Supplemental Material - ja0576637si20060206_084826.pdf
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Additional details
- Eprint ID
- 68532
- Resolver ID
- CaltechAUTHORS:20160620-124352999
- R01 HG003594
- NIH
- R01-CA77373
- NIH
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- PF-03-124-01-GMC
- American Cancer Society
- Mathers Foundation
- CA92584
- NIH
- Created
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2016-06-20Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field