Published February 1966
| public
Journal Article
Effect of phleomycin upon replication of bacteriophage ΦX174
- Creators
- Pitts, John D.
- Sinsheimer, Robert L.
Abstract
Earlier work has shown that the replication of bacteriophage ΦX174 involves three stages of DNA synthesis: (i) the addition of a complementary strand to the single stranded parental DNA (Sinsheimer, Starman, Nagler & Guthrie, 1962) to produce a "replicative form"; (ii) the semi-conservative replication of this replicative form; and (iii) the synthesis of single-stranded DNA for progeny phage by a conservative process (Denhardt & Sinsheimer, 1965b). The addition of chloramphenicol, which inhibits protein synthesis, does not affect stages (i) and (ii) but does prevent stage (iii).
Additional Information
© 1966 Elsevier Ltd. Received 29 November 1965. The gift of phleomycin by Dr Lein, Bristol Laboratories, is gratefully acknowledged. This research was supported, in part, by grant RG6965 from the U.S. Public Health Service.Additional details
- Eprint ID
- 68471
- Resolver ID
- CaltechAUTHORS:20160617-092651406
- RG6965
- U.S. Public Health Service (USPHS)
- Created
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2016-06-17Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field