Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published August 1964 | public
Journal Article

Structure of the DNA of Bacteriophage ΦX174: V. Purine Sequences

Abstract

Methods are described for the preparation of DNA, specifically labeled with carbon-14 in the purines, from bacteriophage ΦX174. This DNA, mixed with unlabeled calf thymus DNA as a carrier, has been degraded to purine tracts by hydrazinolysis and subsequent alkaline hydrolysis. The purine tracts have been separated according to magnitude by chromatography on a column of DEAE-Sephadex, at 55°C in the presence of 7 M-urea.

Additional Information

© 1964 Elsevier Ltd. . , Available online 15 May 2009. We acknowledge the assistance of Mrs. Regine Weil in the growth and purification of the purine-labeled ΦX and of Mrs. Carolyn Nagler in the chromatographic refractlonations, This research was supported, in part, by a grant, RG6965, from the U.S. Public Health Service.

Additional details

Created:
August 19, 2023
Modified:
October 19, 2023