Published December 1973
| public
Journal Article
Replication of Sindbis Virus III. An Electron Microscopic Study of Virus Maturation Using the Surface Replica Technique
Abstract
We have examined surface replicas of chick embryo cells infected with Sindbis virus. In some cells the virus buds over extensive areas of the cell surface, although there are areas where budding virus is clustered and other regions almost devoid of budding figures . In other cells, virus budding occurs primarily or exclusively at the periphery of the monolayer cells. Budding in these cells often occurs in long processes extending from the cell, which have widths from one to several virion diameters and lengths up to 7μm. These processes have also been examined by means of thin sections to visualize their internal features, especially the presence of nucleocapsids in the process of budding.
Additional Information
© 1973 Elsevier Inc. Accepted August 28, 1973. We are grateful to Dr. Jean-Paul Revel for pointing out the advantages of the surface-replica technique to us and for providing instruction and encouragement throughout the course of this project. We are also indebted to Dr. Ross Johnson for suggesting the thin-section experiments and to Mr . Pat Koen for assistance in cutting sections. Edith Lenches and Shaman Christoph provided excellent technical assistance. This research was supported by Grants GM 06905 and AI 10793 from the U.S. Public Health Service and by Grant GB 31763X from the National Science Foundation. One of us (C. R. B.) was supported by U.S. Public Health Service Training Grant GM-86.Additional details
- Eprint ID
- 67890
- DOI
- 10.1016/0042-6822(73)90047-0
- Resolver ID
- CaltechAUTHORS:20160613-145752635
- GM06905
- NIH
- AI 10793
- NIH
- GB 31763X
- NSF
- GM-86
- NIH Predoctoral Fellowship
- Created
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2016-06-13Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field