Fractionation of Nucleic Acid on Benzoylated-Naphthoylated DEAE Cellulose

Abstract

Nucleic acids have been fractionated by column chromatography using methylated albumin kieselguhr (Mandell & Hershey, 1960; Sueoka & Cheng, 1962) cellulose CF-11 (Franklin, 1966), agarose (Erikson & Gordon, 1966) and hydroxyapatite (Muench & Berg, 1966). In this communication we describe the fractionation of nucleic acids on columns of benzoylated-naphthoylated DEAE cellulose (Tener, Gilliam, von Tigerstromm, Millward & Wimmer, 1966) (BNC columns) and show that this fractionation is based on the secondary structure of the nucleic acids.

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