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Published December 19, 1967 | public
Journal Article

Use of Brij lysis as a general method to prepare polyribosomes from Escherichia coli

Abstract

1. A method of preparing polyribosomes from any strain of Escherichia coli is described. 2. The polyribosome patterns obtained vary with the growth rate (growth media) of the cell. When the cells are grown (in rich media) with a short generation time, there are very few 30-S and 50-S ribosome subunits; when the cells are grown (in a poor medium) with a long generation time, the relative proportion of ribosome subunits increases greatly. 3. Under particular growth conditions, the polyribosome pattern varies with the strain of cell used.

Additional Information

© 1967 Elsevier B.V. Received 19 June 1967. This research was supported in part by Grants RG6965 and GM13554 of the U.S. Public Health Service.

Additional details

Created:
August 19, 2023
Modified:
October 18, 2023