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Published August 1955 | Published
Journal Article Open

The Action of Pancreatic Deoxyribonuclease. II. Isomeric Dinucleotides

Abstract

In Paper I (1) methods were described for the isolation of dideoxyribonucleotides from digests of deoxyribonucleic acid (DNA) by pancreatic deoxyribonuclease (DNase). Dinucleotides composed of unlike mononucleotides were obtained as mixtures of the two possible sequential isomers. Methods have now been developed for the analysis of the proportions of the isomers in such mixtures and applied to the dinucleotide fractions obtained by the action of DNase on DNA from calf thymus.

Additional Information

© 1955 American Society for Biochemistry and Molecular Biology. Received for publication, November 15, 1954. The research described in this paper was supported by a grant from the United States Public Health Service.

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