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Published September 1952 | Published
Journal Article Open

A Purification of venom phosphodiesterase

Abstract

For purposes of analysis of polynucleotides, it is desirable to have a phosphodiesterase, substantially free of 5-nucleotidase or other phosphatase activity. The presence of a phosphodiesterase in a wide variety of snake venoms was demonstrated by Gullan and Jackson (1). These venoms were found also to contain a potent 5-nucleotidase, but were free of alkaline phosphatase activity. Hurst and Butler (2) found that certain samples of Russell's viper venom were nearly free of 5-nucleotidase activity, while retaining potent phosphodiesterase action. By a chromatographic procedure, involving the use of cellulose columns, they were able to reduce the 5-nucleotidase activity of rattlesnake venom, relative to its phosphodiesterase activity, and to obtain fractions nearly comparable to the viper venom.

Additional Information

© 1952 American Society for Biochemistry and Molecular Biology. Received for publication, May 5, 1952. This work was supported by a grant from the Rockefeller Foundation.

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Created:
August 19, 2023
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