A Purification of venom phosphodiesterase
- Creators
- Sinsheimer, Robert L.
- Koerner, James F.
Abstract
For purposes of analysis of polynucleotides, it is desirable to have a phosphodiesterase, substantially free of 5-nucleotidase or other phosphatase activity. The presence of a phosphodiesterase in a wide variety of snake venoms was demonstrated by Gullan and Jackson (1). These venoms were found also to contain a potent 5-nucleotidase, but were free of alkaline phosphatase activity. Hurst and Butler (2) found that certain samples of Russell's viper venom were nearly free of 5-nucleotidase activity, while retaining potent phosphodiesterase action. By a chromatographic procedure, involving the use of cellulose columns, they were able to reduce the 5-nucleotidase activity of rattlesnake venom, relative to its phosphodiesterase activity, and to obtain fractions nearly comparable to the viper venom.
Additional Information
© 1952 American Society for Biochemistry and Molecular Biology. Received for publication, May 5, 1952. This work was supported by a grant from the Rockefeller Foundation.Attached Files
Published - J._Biol._Chem.-1952-Sinsheimer-293-6.pdf
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Additional details
- Eprint ID
- 67480
- Resolver ID
- CaltechAUTHORS:20160531-085521801
- Rockefeller Foundation
- Created
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2016-05-31Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field