Published February 18, 1987
| public
Journal Article
Nonenzymatic sequence-specific cleavage of single-stranded DNA to nucleotide resolution. DNA methyl thioether probes
- Creators
- Iverson, Brent L.
-
Dervan, Peter B.
Abstract
We report the sequence-specific cleavage of single-stranded DNA to nucleotide resolution using chemically activated oligodeoxynucleotide methyl thioether hybridization probes (DNAMT). 5-[3-[ [3-(Methylthio)propionyl]amino]-trans-1- propenylldeoxyuridine S'-triphosphate (MT-dUTP) was enzymatically incorporated into an oligonucleotide duplex by using the Klenow fragment of DNA polymerase. Activation with CNBr at 25 OC, pH 5.5, followed by treatment with piperidine produced cleavage on the complementary strand at a single guanine (G) residue to the 5'-side of the modified base.
Additional Information
© 1987 American Chemical Society. Received September 2, 1986. We are grateful to the National Institutes of Health (GM-35724) for support of this research and for a National Research Service Award (T32GM07616) to B.L.I. from the National Institute of General Medical Sciences.Additional details
- Eprint ID
- 67013
- Resolver ID
- CaltechAUTHORS:20160511-145020102
- GM-35724
- NIH
- T32GM07616
- NIH Predoctoral Fellowship
- Created
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2016-05-19Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field