Cooperative site specific binding of oligonucleotides to duplex DNA
- Creators
- Strobel, Scott A.
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Dervan, Peter B.
Abstract
Cooperative interactions between DNA binding ligands are critical to their specificity, affinity, and biological activity. Triple helix formation by oligonucleotides is the most powerful chemical approach to date for the sequence-specific recognition of double helical DNA. Hoogsteen hydrogen bonded base triplets, TAT and C+GC, result from pyrimidine oligonucleotides binding site specifically to purine duplex sequences. In the triple helical model, a binding site size of 18 purine base pairs affords 36 discrete sequence-specific hydrogen bonds for recognition of DNA in the major groove. As a possible mechanism for improving the specificity of triple helix formation, we tested whether oligonucleotides could cooperatively bind to a double-stranded DNA template.
Additional Information
© 1989 American Chemical Society. Received March 20, 1989. Acknowledgment. This work was supported in part by the National Institutes of Health (GM-35724) and the Caltech Consortium in Chemistry and Chemical Engineering (Founding members: E. I. du Pont de Nemours & Co., Inc., Eastman Kodak Company, Minnesota Mining and Manufacturing Company, and Shell Oil Company Foundation).Additional details
- Eprint ID
- 66962
- DOI
- 10.1021/ja00200a073
- Resolver ID
- CaltechAUTHORS:20160510-155444926
- GM-35724
- NIH
- Caltech Consortium in Chemistry and Chemical Engineering
- Created
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2016-05-19Created from EPrint's datestamp field
- Updated
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2021-11-11Created from EPrint's last_modified field