Adenine-specific DNA chemical sequencing reaction
- Creators
- Iverson, Brent L.
-
Dervan, Peter B.
- Other:
- Wu, Ray
Abstract
DNA sequence determination according to the method of Maxam and Gilbert utilizes base-specific chemical modification reactions followed by a workup that causes cleavage of the sugar-phosphate backbone at the site of the modified base. Reactions have been reported that are capable of selective cleavage of DNA at G, G + A, A > G, A > C, C, C + T, and T residues. We describe here a simple protocol specific for adenine (A) that may be a useful addition to current chemical sequencing reactions. Reaction of DNA with K_2PdC1_4 at pH 2.0 followed by heating in the presence of piperidine produces an A-specific DNA cleavage reaction. The K_2PdCI_4 reaction involves selective depurination at adenine, affording an excision reaction analogous to the other chemical DNA sequencing reactions.
Additional Information
© 1993 Academic Press. We are grateful to the American Cancer Society for support of this research and for a National Research Service Award to B.L.I. from the National Institute of General Medical Sciences.Additional details
- Eprint ID
- 66930
- DOI
- 10.1016/0076-6879(93)18017-7
- Resolver ID
- CaltechAUTHORS:20160510-140053565
- American Cancer Society
- NIH Predoctoral Fellowship
- Created
-
2016-05-19Created from EPrint's datestamp field
- Updated
-
2021-11-11Created from EPrint's last_modified field
- Series Name
- Methods in Enzymology
- Series Volume or Issue Number
- 218