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Published October 19, 1993 | public
Journal Article

NMR structural studies on a nonnatural deoxyribonucleoside which mediates recognition of GC base pairs in pyrimidine•purine•pyrimidine DNA triplexes

Abstract

As a part of our ongoing efforts to define the structural aspects of unusual pairing alignments in DNA triplexes by nuclear magnetic resonance spectroscopy, we have examined the structural role of a nonnatural deoxyribonucleoside, P1, that has been shown to mediate the recognition of GC base pairs in pyrimidine-purine-pyrimidine DNA triplexes [Koh, J.S., & Dervan, P.B. (1992) J. Am. Chem Soc. 114, 1470]. A qualitative interpretation of the NMR data indicates that this analog of protonated cytosine is readily accommodated in the third strand segment of an intramolecular triplex system. Furthermore, the observed NOE patterns position the imino and amino protons of P1 opposite the N^7 and O^6 atoms of guanine, respectively, consistent with the previously proposed pairing scheme.

Additional Information

© 1993 American Chemical Society. Received June 4, 1993; Revised Manuscript Received July 30, 1993. Abstract published in Advance ACS Abstracts, September 15, 1993. We are grateful to the National Institutes of Health (GM 34504) for research support to D.J.P., the Office of Naval Research for research support to P.B.D., and the National Science Foundation for a predoctoral fellowship to ESP. We thank Dr. Steven Woski for a supply of 2'-deoxy-5-methylcytidinederivatized controlled pore glass. The NMR spectrometers were purchased from funds donated by the Robert Woods Johnson Trust toward setting up an NMR Center in the Basic Medical Sciences at Columbia University. We acknowledge the use of the Molecular Modeling Facility for Molecular Biology at Columbia University, supported in part by NSF Grant DIR-8720229.

Additional details

Created:
August 20, 2023
Modified:
October 18, 2023