A Versatile Mismatch Recognition Agent: Specific Cleavage of a Plasmid DNA at a Single Base Mispair
Abstract
[Rh(bpy)_2(chrysi)]^(3+) is a novel, sterically bulky DNA intercalator that has been designed to bind specifically in the destabilized regions near DNA base mismatches and, upon photoactivation, to cleave the DNA backbone. Here the molecule is shown to be both a general and remarkably specific mismatch recognition agent. Specific DNA cleavage is observed at over 80% of mismatch sites in all the possible single base pair sequence contexts around the mispaired bases. Moreover, the complex is highly site-specific; it is shown to recognize and photocleave at a single base mismatch in a 2725 base pair linearized plasmid heteroduplex. Sterically demanding intercalators such as [Rh(bpy)_2(chrysi)]^(3+) may have application both in mutation detection systems and as mismatch-specific chemotherapeutic agents.
Additional Information
© 1999 American Chemical Society. Received February 3, 1999; Revised Manuscript Received February 26, 1999. Publication Date (Web): March 23, 1999. Support from the National Institutes of Health (GM33309) is gratefully acknowledged. B.A.J. acknowledges the NSF and the Parsons Foundation for predoctoral fellowships, and V.Y.A. acknowledges the HHMI and the Caltech SURF program for sponsorship of a summer undergraduate research fellowship.Additional details
- Eprint ID
- 65991
- DOI
- 10.1021/bi990255t
- Resolver ID
- CaltechAUTHORS:20160407-115523781
- GM33309
- NIH
- NSF
- Ralph M. Parsons Foundation
- Howard Hughes Medical Institute (HHMI)
- Caltech Summer Undergraduate Research Fellowship (SURF)
- Created
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2016-04-11Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field