Cardiotrophin-1 Induces the Same Neuropeptides in Sympathetic Neurons as Do Neuropoietic Cytokines
Abstract
Cardiotrophin-1 (CT-1) was cloned from mouse embryoid body for its ability to induce growth of heart cells. Predictions of its secondary structure indicate that CT-1 belongs to a family of cytokines with a four-helical bundle structure, and sequence comparisons reveal a weak homology to leukemia inhibitory factor (LIF) and ciliary neurotrophic factor (CNTF). Using a reverse transcriptase-polymerase chain reaction assay with rat sympathetic neuron cultures, we find that CT-1 induces and suppresses the expression of the same set of neuropeptide and neurotransmitter synthetic enzyme mRNAs as do LIF and CNTF. In addition, the effects of CT-1 and LIF are not additive, and CT-1 does not require a GPI-linked component to mediate its actions. Our functional data confirm that CT-1 is a member of the neuropoietic cytokine family and suggest that the CT-1 receptor complex contains the gp130 signal transducing component.
Additional Information
© 1997 International Society for Neurochemistry. Received May 9, 1997; revised manuscript received July 14, 1997; accepted July 14, 1997. Article first published online: 18 Nov 2002. We thank M. J. Fann for assistance with RT-PCR. This study was supported by a grant from NINDS to P.H.P. and a Gosney Fellowship to J.-G.C.Additional details
- Eprint ID
- 65978
- DOI
- 10.1046/j.1471-4159.1997.69062278.x
- Resolver ID
- CaltechAUTHORS:20160407-073653884
- National Institute of Neurological Disorders and Stroke (NINDS)
- Caltech Gosney Fellowship
- Created
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2016-04-07Created from EPrint's datestamp field
- Updated
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2022-10-04Created from EPrint's last_modified field