Partial N-terminal amino acid sequences of three nonstructural proteins of two flaviviruses
Abstract
Partial N-terminal amino acid sequences for the three largest nonstructural proteins of two flaviviruses, yellow fever virus and St. Louis encephalitis virus, have been obtained. The determined sequences of these proteins exhibit significant amino acid sequence homology, and allow the positioning of these three nonstructural proteins in the polyprotein sequence deduced from the nucleotide sequence of yellow fever virus (C. M. Rice, E. M. Lenches, S. R. Eddy, S. J. Shin, R. L. Sheets, and J. H. Strauss, 1985, Science 229, 726-733.) The deduced start points support the hypothesis that the N terminus of nonstructural glycoprotein NSl results from cleavage by signalase, whereas the N termini of NS3 and NS5 result from cleavages following double basic residues that are flanked by amino acids with short side chains.
Additional Information
© 1986 Academic Press, Inc. Received November 12, 1985; accepted January 22, 1986 We gratefully acknowledge the competent technical assistance of E. Lenches. We are grateful to E. Strauss for help with the manuscript and in producing the figures. The computer facility of L. Hood and the programs written by T. Hunkapiller were of great value in producing the figures. This work was supported by Grants AI 20612, AI 10793 from NIH and Grant DMB 83-16856 from NSF. R.A. was the recipient of a training fellowship from the Swiss National Foundation.Additional details
- Eprint ID
- 65557
- DOI
- 10.1016/0042-6822(86)90098-X
- Resolver ID
- CaltechAUTHORS:20160321-150516329
- AI 20612
- NIH
- AI 10793
- NIH
- DMB 83-16856
- NSF
- Swiss National Foundation
- Created
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2016-03-22Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field