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Published July 1995 | public
Journal Article

Developmental Utilization of SpP3A1 and SpP3A2: Two Proteins Which Recognize the Same DNA Target Site in Several Sea Urchin Gene Regulatory Regions

Abstract

SpP3A1 and SpP3A2 are DNA-binding proteins that interact specifically with the same target sites in the regulatory domains of the Strongylocentrotus purpuratus CyIIIa gene and also of several other known genes. In this work we used antibodies raised against recombinant P3A1 and P3A2 to quantitate these transcription factors in eggs and in the nuclear compartments of embryos of various stages. Both proteins are present in unfertilized eggs, and both enter the embryonic nuclei early in development, but only P3A2 remains present in nuclei at functional concentrations beyond the early gastrula stage. Combined with earlier measurements of P3A site binding at cleavage stages, these measurements show that P3A1 would be replaced by P3A2 at target sites in genes regulated by these factors.

Additional Information

© 1995 Academic Press, Inc. Accepted April 19, 1995. We thank Dr. Scott E. Fraser for critical reading of the manuscript. This research was supported by the NIH Grant HD-05753. R. W. Z. was supported by NIH Training Grant GM-07616 and by the ONR ASSERT Program (N00014-93-1-1400).

Additional details

Created:
August 20, 2023
Modified:
October 18, 2023