Delayed Formation of the Floor Plate after Ablation of the Avian Notochord
Abstract
We have examined the long-term effects of notochord ablation at chick stages 9–10 on formation of the floor plate and motor neurons. Although missing or reduced 2 days postablation, the floor plate and motor neurons were morphologically normal by 4 postoperative days. When isolated whole or ventral, but not lateral, neural plate fragments from stage 9 embryos were cultured for 4 days in collagen gels, floor plate and neural markers were observed. Our results suggest that floor plate and motor neurons can form in a delayed fashion in vivo after notochord ablation and in vitro from isolated neural plates. This suggests that either there is an early induction of floor plate by the chordamesoderm of Hensen's node, or only limited interactions between the neural plate and notochord immediately after neurulation are required for floor plate determination.
Additional Information
© 1993 Cell Press. Received 17 May 1993, Revised 20 October 1993. We would like to thank Drs. S. Fraser, M. Selleck, T. Scherson, J. Sechrist, and the reviewers for helpful comments on the manuscript. We are indebted to Dr. M. Selleck for his skillful technical assistance with the cover artwork. We are grateful to Drs. C. Stern and A. Lumsden for showing us the notochord ablation technique. We thank Dr. M. Tessier-Lavigne and S. Colamarino for help with the collagen gel embedding procedure and Dr. S. Chang for DM-2 antibodies. SC-1, FP-1, and Not-1 antibodies were obtained from Developmental Studies Hybridoma Bank maintained by Johns Hopkins University and the University of Iowa under contract number NO1-HD-2-3144 from the NICHD. This work was supported by USPHS HD-25138. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC Section 1734 solely to indicate this fact.Additional details
- Eprint ID
- 65223
- DOI
- 10.1016/0896-6273(93)90227-I
- Resolver ID
- CaltechAUTHORS:20160309-072134200
- NO1-HD-2-3144
- National Institute of Child Health and Human Development (NICHD)
- HD-25138
- NIH
- Created
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2016-03-09Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field