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Published September 13, 1996 | public
Journal Article

Neuronal expression of leukemia inhibitory factor (LIF) in the rat brain

Abstract

Very little is known about the action of the leukemia inhibitory factor (LIF) in the central nervous system. To analyze LIF expression in adult rat brain, we used non-radioactive in situ hybridization with digoxigenin-labeled RNA probes, in combination with immunohistochemistry to identify the cell types expressing LIF mRNA. Hippocampal pyramidal cells, hilar interneurons and granule cells of the dentate gyrus display strong hybridization signals, while neurons of the cerebral cortex, cerebellum, cholinergic basal forebrain nuclei, and the striatum are labeled as well. The most prominent expression in cerebral cortex is in layers IV and VI. Glial fibrillary acidic protein-positive astrocytes did not display a detectable LIF hybridization signal suggesting that LIF mRNA is predominantly expressed in neurons in the normal, adult rat brain, although a presence in resting microglial cells cannot be excluded.

Additional Information

© 1996 Elsevier Ireland Ltd. Received 8 July 1996; revised version received 14 August 1996; accepted 14 August 1996. This work was supported by a grant of the NIH to P.H.P and by the Bundesministerium für Forschung und Technik, grant FKZ 01 ZZ 9130 to R.S.R.L. is a recipient of a stipend from the Studienstiftung des Deutschen Volkes e.V., and R.A.G. is supported by the Swiss National Science Foundation and the Roche Research Foundation. In addition, we gratefully acknowledge the University of Leipzig Medical School, Germany for covering travel expenses, and Dr. H. Govan for critical comments.

Additional details

Created:
August 22, 2023
Modified:
October 17, 2023