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Published March 2016 | Accepted Version
Journal Article Open

A novel subset of enteric neurons revealed by ptf1a:GFP in the developing zebrafish enteric nervous system

Abstract

The enteric nervous system, the largest division of the peripheral nervous system, is derived from vagal neural crest cells that invade and populate the entire length of the gut to form diverse neuronal subtypes. Here, we identify a novel population of neurons within the enteric nervous system of zebrafish larvae that express the transgenic marker ptf1a:GFP within the midgut. Genetic lineage analysis reveals that enteric ptf1a:GFP+ cells are derived from the neural crest and that most ptf1a:GFP+ neurons express the neurotransmitter 5HT, demonstrating that they are serotonergic. This transgenic line, Tg(ptf1a:GFP), provides a novel neuronal marker for a subpopulation of neurons within the enteric nervous system, and highlights the possibility that Ptf1a may act as an important transcription factor for enteric neuron development.

Additional Information

© 2016 Wiley Periodicals, Inc. Accepted manuscript online: 10 FEB 2016 10:37PM EST. Manuscript Accepted: 9 FEB 2016. Manuscript Revised: 4 JAN 2016. Manuscript Received: 7 SEP 2015. We thank Ryan Anderson (Indiana University School of Medicine) for sharing the Tg(ptf1a:GFP) line and Robert Kelsh (University of Bath) for sharing the Tg(-4725sox10:Cre;elf1a:loxp-GFP-loxp-dsRedpA) line. We thank Crystal Rogers and Stephen Green for helpful discussions, Yuk Fai Leung (Purdue University) for pCS2-ptf1a construct and Martha Henderson and David Mayorga for fish care. Research was supported by grants from NIH DE024157 to M.E.B., from NIH F32 HD080343 to R.A.U. and a Burroughs Wellcome Fund Postdoctoral Enrichment Program Award to R.A.U.

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August 22, 2023
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