High-Throughput Method for Automated Colony and Cell Counting by Digital Image Analysis Based on Edge Detection
- Creators
- Choudhry, Priya
Abstract
Counting cells and colonies is an integral part of high-throughput screens and quantitative cellular assays. Due to its subjective and time-intensive nature, manual counting has hindered the adoption of cellular assays such as tumor spheroid formation in high-throughput screens. The objective of this study was to develop an automated method for quick and reliable counting of cells and colonies from digital images. For this purpose, I developed an ImageJ macro Cell Colony Edge and a CellProfiler Pipeline Cell Colony Counting, and compared them to other open-source digital methods and manual counts. The ImageJ macro Cell Colony Edge is valuable in counting cells and colonies, and measuring their area, volume, morphology, and intensity. In this study, I demonstrate that Cell Colony Edge is superior to other open-source methods, in speed, accuracy and applicability to diverse cellular assays. It can fulfill the need to automate colony/cell counting in high-throughput screens, colony forming assays, and cellular assays.
Additional Information
© 2016 Priya Choudhry. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published: February 5, 2016. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. The IMJ macro, CellProfiler pipelines, and images are currently available from https://sourceforge.net/projects/cell-colony-edge/files/. Funding: The authors have no support or funding to report. The authors have declared that no competing interests exist. Author Contributions: Conceived and designed the experiments: PC. Performed the experiments: PC. Analyzed the data: PC. Contributed reagents/materials/analysis tools: PC. Wrote the paper: PC. Cellular assay, imaging, analysis, writing macro: PC.Attached Files
Published - journal.pone.0148469.pdf
Supplemental Material - journal.pone.0148469.s001.PDF
Supplemental Material - journal.pone.0148469.s002.PDF
Supplemental Material - journal.pone.0148469.s003.PDF
Supplemental Material - journal.pone.0148469.s004.PDF
Supplemental Material - journal.pone.0148469.s005.TIFF
Supplemental Material - journal.pone.0148469.s006.TIF
Supplemental Material - journal.pone.0148469.s007.TIF
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Additional details
- PMCID
- PMC4746068
- Eprint ID
- 64499
- Resolver ID
- CaltechAUTHORS:20160216-103546006
- Created
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2016-02-17Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field