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Published July 27, 1970 | public
Journal Article

Novel protein composition of a bacterial membrane

Abstract

The ghosts of a Bacillus sp. (Bacillus PP) isolated from a culture of Bacillus megaterium KM were dissociated by treatment with urea-sodium deoxycholate and fractionated by gel filtration. Examination of the protein composition of the unfractionated and the fractionated membrane components by disc gel electrophoresis under dissociating conditions revealed the presence of a protein band of MW 32,000 that comprised at least 90% of the total protein of the ghosts. An electrophoretically identical protein was found to be a relatively minor component of the ghosts of Bacillus megaterium KM.

Additional Information

© 1970 Published by Elsevier Inc. Received 11 June 1970. The authors wish to express their thanks to Betty Earles and Natalie Wehman for their assistance with these experiments.

Additional details

Created:
August 19, 2023
Modified:
October 17, 2023