DNA sequence organization in the genomes of five marine invertebrates
Abstract
The arrangement of repetitive and non-repetitive sequence was studied in the genomic DNA of the oyster (Crassostrea virginica), the surf clam (Spisula solidissima), the horseshoe crab (Limulus polyphemus), a nemertean worm (Cerebratulus lacteus) and a jellyfish (Aurelia aurita). Except for the jellyfish these animals belong to the protostomial branch of animal evolution, for which little information regarding DNA sequence organization has previously been available. The reassociation kinetics of short (250–300 nucleotide) and long (2,000–3,000 nucleotide) DNA fragments was studied by the hydroxyapatite method. It was shown that in each case a major fraction of the DNA consists of single copy sequences less than about 3,000 nucleotides in length, interspersed with short repetitive sequences. The lengths of the repetitive sequences were estimated by optical hyperchromicity and S1 nuclease measurements made on renaturation products. All the genomes studied include a prominent fraction of interspersed repetitive sequences about 300 nucleotides in length, as well as longer repetitive sequence regions.
Additional Information
© 1975 Springer. Received and accepted April 11, 1975 by J. G. Gall. Ready for press April 16, 1975. We are pleased to acknowledge the expert technical assistance of Ms. Margaret Chamberlin and Ms. Denise Painchaud. This research was supported by NIH Training Grant No. HD-00014 to the Marine Biological Laboratory's Embryology Course and by NIH Grant No. GM-20927.Additional details
- Eprint ID
- 63009
- Resolver ID
- CaltechAUTHORS:20151216-170610841
- HD-00014
- NIH
- GM-20927
- NIH
- Created
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2016-01-19Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field