Persistence of maternal RNA in Engystomops embryos
Abstract
Females of the neotropic anuran Engystomops pustulosus were injected with 3H-uridine and produced eggs containing RNA labeled during synchronous oogenesis. The >4S, labeled RNA extracted from these eggs, and from embryos which developed from them, was hybridized with an isolated repetitive fraction of Engystomops DNA, and the hybrids were assayed on hydroxyapatite columns. About 1% of the egg RNA is transcribed from DNA sequences included in this fraction. Hybridization of labeled ribosomal RNA was minimized by the addition of excess unlabeled ribosomal RNA extracted from Xenopus ovaries. The labeled RNA of embryos up to the tadpole stage was identified as maternal transcript by competition experiments with unlabeled Engystomops oocyte RNA. It was found that maternally-labeled RNA is retained in embryos at least up to the tadpole stage.
Additional Information
© 1973 Wiley-Liss, Inc. This research was supported by the National Institute of Child Health and Development grant HD-05753, and National Science Foundation grant GB-33441X. We acknowledge the expert technical assistance of Ms. Margaret Chemberlin, and of Ms. Jane Rigg and Mr. Charles Collett in the care of the Engystomops colony and collection of embryos. Special thanks are due to Drs. Roy J. Britten and Michael J. Smith for their invaluable assistance in the collection of toads in Panama.Additional details
- Eprint ID
- 63008
- Resolver ID
- CaltechAUTHORS:20151216-165729835
- HD-05753
- NIH
- GB-33441X
- NSF
- Created
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2016-01-27Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field