Process of Infection with φX174: Effect of Exonucleases on the Replicative Form

Creators: Burton, Alice; Sinsheimer, Robert L.

Abstract

When a preparation containing the DNA of the bacteriophage φX174 in its replicative form together with DNA from Escherichia coli is treated with exonucleases, the replicative form retains its characteristic physical properties, while the bacterial DNA is degraded. The evidence suggests that the mature replicative form exists as a closed ring.

Additional Information

© 1963 American Association for the Advancement of Science. 26 August 1963. We thank Dr. C. C. Richardson and Dr. I. R. Lehman for generous gifts of the E. coli exonuclease III and the E. coli exonuclease I. One of us (A.B.) has been a postdoctoral fellow of the U.S. Public Health Service. This research has been supported in part by grant RG-6965 from the U.S. Public Health Service.

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Process of Infection with φX174: Effect of Exonucleases on the Replicative Form

Abstract

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