Published December 16, 1977
| public
Journal Article
Localization of the Globin Gene in the Template Active Fraction of Chromatin of Friend Leukemia Cells
- Creators
- Wallace, R. Bruce
- Dube, Shyam K.
- Bonner, James
Abstract
Friend leukemia cell chromatin has been fractionated into template active and inactive components. The globin gene sequence is associated with the template active component both prior to and after the cells are induced with dimethyl sulfoxide to synthesize hemoglobin and therefore appears to be in an active configuration in uninduced as well as in induced Friend leukemia cells. In cells which have lost the ability to produce hemoglobin, the globin gene sequence is not associated with the template active fraction of chromatin. These results demonstrate the success of the fractionation procedure.
Additional Information
© 1977 American Association for the Advancement of Science. 10 March 1977; revised 29 July 1977. We thank I. B. Pragnell and W. Ostertag for a gift of globin cDNA and helpful discussions. This research was supported by PHS grant GM-13762. S.K.D. has been supported as a Sherman Fairchild Distinguished Scholar at the California Institute of Technology. R.B.W. is the recipient of a Medical Research Council of Canada fellowship.Additional details
- Eprint ID
- 62149
- DOI
- 10.1126/science.270812
- Resolver ID
- CaltechAUTHORS:20151117-080411815
- GM-13762
- Public Health Service
- Caltech
- Medical Research Council of Canada
- Created
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2015-11-17Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field