Optically transparent multi-suction electrode arrays
Abstract
Multielectrode arrays (MEAs) allow for acquisition of multisite electrophysiological activity with submillisecond temporal resolution from neural preparations. The signal to noise ratio from such arrays has recently been improved by substrate perforations that allow negative pressure to be applied to the tissue; however, such arrays are not optically transparent, limiting their potential to be combined with optical-based technologies. We present here multi-suction electrode arrays (MSEAs) in quartz that yield a substantial increase in the detected number of units and in signal to noise ratio from mouse cortico-hippocampal slices and mouse retina explants. This enables the visualization of stronger cross correlations between the firing rates of the various sources. Additionally, the MSEA's transparency allows us to record voltage sensitive dye activity from a leech ganglion with single neuron resolution using widefield microscopy simultaneously with the electrode array recordings. The combination of enhanced electrical signals and compatibility with optical-based technologies should make the MSEA a valuable tool for investigating neuronal circuits.
Additional Information
© 2015 Nagarah, Stowasser, Parker, Asari and Wagenaar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Received: 07 September 2015; Accepted: 02 October 2015; Published: 20 October 2015. We are grateful to the following people: James Heath for use of his cleanroom facility; Jen-Kan Yu, Slobodan Mitrovic, and the UCLA Nanoelectronics Research Facility staff, especially Joe Zendejas and Tom Lee, for their support with device fabrication; Markus Meister for aid in the retina experiments; Henry Lester and Bruce Cohen for the use of lab space and equipment for slice recordings; Bill Eggers for discussions on electrochemical impedance analysis; Karl-Heinz Boven and Frank Hofmann for discussions on slice recordings and perforated MEAs; Eugene Lubenov and Julie Miwa for further discussions on slice recordings; Habib Ahmad for help with 3D renderings. Funding for this work was provided by the Broad Foundations, by a Career Award at the Scientific Interface (#1007977) from the Burroughs Wellcome Fund to DW, and by funds provided by the Regents of the University of California, Tobacco-Related Diseases Research Program (grant no 22DT-0008) to RP. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.Attached Files
Published - fnins-09-00384.pdf
Supplemental Material - presentation_1.pdf
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Additional details
- PMCID
- PMC4611137
- Eprint ID
- 61993
- Resolver ID
- CaltechAUTHORS:20151109-105331416
- Eli and Edythe Broad Foundation
- 1007977
- Burroughs Wellcome Fund
- Regents of the University of California
- 22DT-0008
- California Tobacco-Related Disease Research Program
- Created
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2015-11-09Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field