In vivo sub-femtoliter resolution photoacoustic microscopy with higher frame rates
Abstract
Microscopy based on non-fluorescent absorption dye staining is widely used in various fields of biomedicine for 400 years. Unlike its fluorescent counterpart, non-fluorescent absorption microscopy lacks proper methodologies to realize its in vivo applications with a sub-femtoliter 3D resolution. Regardless of the most advanced high-resolution photoacoustic microscopy, sub-femtoliter spatial resolution is still unattainable, and the imaging speed is relatively slow. In this paper, based on the two-photon photoacoustic mechanism, we demonstrated a in vivo label free laser-scanning photoacoustic imaging modality featuring high frame rates and sub-femtoliter 3D resolution simultaneously, which stands as a perfect solution to 3D high resolution non-fluorescent absorption microscopy. Furthermore, we first demonstrated in vivo label-free two-photon acoustic microscopy on the observation of non-fluorescent melanin distribution within mouse skin.
Additional Information
© 2015 Macmillan Publishers Limited. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ Received: 28 May 2015. Accepted: 18 September 2015. Published online: 21 October 2015. We would like to thank Prof. Chen-Tung Yen for assistance with samples and Zurich Instruments for support. Also, we thank Dr. Wei-Hung Weng for his help of melanin validation in acquired images. This work was sponsored by Ministry of Education and Molecular Imaging Center of National Taiwan University, the National Health Research Institute of Taiwan under NHRI-EX104-9936EI and by the Ministry of Science and Technology of Taiwan under MOST 103-2221-E-002-137-MY3. Contributions: C.K.S. initiated the concept and conducted the experiments. S.Y.L. designed the microscopy system and analyzed all data. S.Y.L., Y.H.L., K.C.H., Y.H.C. and T.F.T. performed the experiments. S.Y.L. and C.K.S. wrote this manuscript. The authors declare no competing financial interests.Attached Files
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Additional details
- PMCID
- PMC4614074
- Eprint ID
- 61542
- Resolver ID
- CaltechAUTHORS:20151027-093111090
- Ministry of Education (Taipei)
- National Taiwan University
- NHRI-EX104-9936EI
- National Health Research Institute of Taiwan
- MOST 103-2221-E-002-137-MY3
- Ministry of Science and Technology (Taipei)
- Created
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2015-10-27Created from EPrint's datestamp field
- Updated
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2022-05-19Created from EPrint's last_modified field