Visualization of cellulose synthases in Arabidopsis secondary cell walls
Abstract
Cellulose biosynthesis in plant secondary cell walls forms the basis of vascular development in land plants, with xylem tissues constituting the vast majority of terrestrial biomass. We used plant lines that contained an inducible master transcription factor controlling xylem cell fate to quantitatively image fluorescently tagged cellulose synthase enzymes during cellulose deposition in living protoxylem cells. The formation of secondary cell wall thickenings was associated with a redistribution and enrichment of CESA7-containing cellulose synthase complexes (CSCs) into narrow membrane domains. The velocities of secondary cell wall–specific CSCs were faster than those of primary cell wall CSCs during abundant cellulose production. Dynamic intracellular of endomembranes, in combination with increased velocity and high density of CSCs, enables cellulose to be synthesized rapidly in secondary cell walls.
Additional Information
© 2015 American Association for the Advancement of Science. Received 5 June 2015; accepted 26 August 2015. Arabidopsis plants containing RFP::TUB6 were the kind gift of C. Ambrose and G. O. Wasteneys. Supported by CREATE and Discovery grants (L.S. and S.D.M.); Canadian Natural Sciences and Engineering Research Council doctoral postgraduate scholarships (Y.W., M.J.M., and L.M.M.); NSF grant MCB-1158372 (D.W.E. and H.N.C.); and Japan Society for the Promotion of Science KAKENHI grants 24114002 and 25291062 (T.D.). We thank the technical support staff of the UBC Bioimaging Facility and R. White for statistical advice. The pTA7001 plasmid (VP16-GR vector) is available from N.-H. Chua of The Rockefeller University under a material transfer agreement; the VND7-GR line is available fromT.D.; and the GFP::CESA3 and YFP::CESA7 VND7-GR lines are freely available from L.S. or S.D.M. for noncommercial purposes under a materials transfer agreement on the GVG system with N.-H. Chua. Further data are reported in the supplementary materials.Attached Files
Supplemental Material - Watanabe.SM.pdf
Supplemental Material - aac7446s1.mov
Supplemental Material - aac7446s2.avi
Supplemental Material - aac7446s3.mov
Supplemental Material - aac7446s4.mov
Supplemental Material - aac7446s5.mov
Supplemental Material - aac7446s6.mov
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Additional details
- Eprint ID
- 61260
- Resolver ID
- CaltechAUTHORS:20151019-134150332
- CREATE
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- MCB-1158372
- NSF
- 24114002
- Japan Society for the Promotion of Science (JSPS)
- 25291062
- Japan Society for the Promotion of Science (JSPS)
- Created
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2015-10-19Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field