Published November 1959
| public
Journal Article
Peptide separation by two-dimensional chromatography and electrophoresis
Abstract
The separation of peptides from controlled proteolytic digests by two-dimensional paper chromatography and electrophoresis is finding wide application to studies of protein structure. With this method it is possible to compare digestion mixtures from different proteins and to detect differences as slight as the replacement of a single amino acid. Such differences are of importance in the comparison of homologous proteins from different animal and plant species, and in the study of genetically determined changes in protein structure, such as those resulting from a single gene mutation.
Additional Information
© 1959 American Society for Biochemistry and Molecular Biology. (Received for publication, June 25, 1959) The authors would like to thank Dr. Edgar Haber and Mr. Richard Stroud for their help in the design and construction of the Lucite tanks.Additional details
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- CaltechAUTHORS:20150811-123906130
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