Chemical synthesis of restriction enzyme recognition sites useful for cloning

Creators: Scheller, Richard H.; Dickerson, Richard E.; Boyer, Herbert W.; Riggs, Arthur D.; Itakura, Keiichi

Abstract

By a triester chemical synthesis method, three decameric DNA's have been made; these act as substrates for several restriction endonucleases, including Eco RI, Bam I, and Hind III. These homogenous decamers form duplexes that can be efficiently blunt-end ligated to themselves or to other DNA molecules by the action of T4 DNA ligase and thus are useful tools for molecular cloning experiments.

Additional Information

© 1977 American Association for the Advancement of Science. Received 4 February 1977. We thank J. Rosenberg and W. Gilbert for their stimulating contributions to the development of the linker concept. We also thank A. S. Lee, H. Heyneker, and J. Shine for help in demonstrating some of the enzyme reactions discussed in this report; and L. Shively for technical help. Supported by NIH grants GM-12121 and HD-04420, NSF grants PCM75-05886 and GB-26517, and an NIH predoctoral traineeship to R.H.S. This is contribution No. 5457 from the Norman W. Church Laboratory of Chemical Biology, California Institute of Technology.

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Chemical synthesis of restriction enzyme recognition sites useful for cloning

Abstract

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