Negative and positive selection of antigen-specific cytotoxic T lymphocytes affected by the α3 domain of MHC I molecules
Abstract
THE α1 and α2 domains of major histocompatibility complex (MHC) class I molecules function in the binding and presentation of foreign peptides to the T-cell antigen receptor and control both negative and positive selection of the T-cell repertoire. Although the α3 domain of class I is not involved in peptide binding, it does interact with the T-cell accessory molecule, CDS. CDS is important in the selection of T cells as anti-CDS antibody injected into perinatal mice interfers with this process. We previously used a hybrid class I molecule with the α1/α2 domains from L^d and the α3 domain from Q7^b and showed that this molecule binds an L^d-restricted peptide but does not interact with CD8-dependent cytotoxic T lymphocytes. Expression of this molecule in transgenic mice fails to negatively select a subpopulation of anti-L^d cytotoxic T lymphocytes. In addition, positive selection of virus-specific L^d-restricted cytotoxic T lymphocytes does not occur. We conclude that besides the α1/α2 domains of class I, the α3 domain plays an important part in both positive and negative selection of antigen-specific cells.
Additional Information
© 1991 Nature Publishing Group. Received 26 March; Accepted 4 July 1991. This work was supported by an NIH grant (J.F.). We thank M. Zuniga for the L^d gene. G. D. Senn Ill for technical assistance, and B. Washington for secretarial help.Additional details
- Eprint ID
- 57924
- DOI
- 10.1038/352718a0
- Resolver ID
- CaltechAUTHORS:20150601-142904818
- NIH
- Created
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2015-06-01Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field