Published February 18, 1994
| public
Journal Article
Progress toward an Antibody Glycosidase
Abstract
The piperidine-based hapten 1 generates monoclonal antibodies that catalyze the hydrolysis of substrate 2 at pH5.5 (k_(cal) = 0.904 h^(−1), K_M = 324 μM). Hapten 1 is a competitive inhibitor of the antibody-catalyzed reaction. These results are significant for the development of antibodies for the sequence-specific hydrolysis of oligosaccharides.
Additional Information
© 1994 VCH Verlagsgesellschaft mbH. Received September 20, 1993. This work was supported by the Office of Naval Research (Grant No. N00014-91-J-I 130) and the Assistant Secretary for Conservation and Renewable Energy, Advanced Industrial Concepts Division of the U.S. Department of Energy (Contract No. DE-AC03-76SF00098). L C. H. is supported by a National Science Foundation Graduate Fellowship. We thank Holly C. Wessling for her assistance.Additional details
- Eprint ID
- 57659
- DOI
- 10.1002/anie.199403391
- Resolver ID
- CaltechAUTHORS:20150519-122534441
- N00014-91-J-1130
- Office of Naval Research (ONR)
- DE-AC03-76SF00098
- Department of Energy (DOE)
- NSF Graduate Research Fellowship
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2015-05-19Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field