Role for a Xenopus Orc2-related protein in controlling DNA replication
Abstract
The six-subunit origin recognition complex (ORC) is essential for the initiation of DNA replication at specific origins in the budding yeast Saccharomyces cerevisiae. An important issue is whether DNA replication in higher eukaryotes, in which the characteristics of replication origins are poorly defined, occurs by an ORC-dependent mechanism. We have identified a Xenopus laevis Orel-related protein (XORC2) by its ability to rescue a mitotic-catastrophe mutant of the fission yeast Schizosaccharomyces pombe. We show that immunodepletion of XORC2 from Xenopus egg extracts abolishes the replication of chromosomal DNA but not elongation synthesis on a single-stranded DNA template. Indirect immunofluorescence indicates that XORC2 binds to chromatin well before the commencement of DNA synthesis, and even under conditions that prevent the association of replication licensing factor(s) with the DNA. These findings suggest that Orc2 plays an important role at an early step of chromosomal replication in animal cells.
Additional Information
© 1996 Nature Publishing Group. Received 25 September; accepted 4 December 1995. We thank T. R. Coleman and A. Kumagai for their contributions; our colleagues for comments on the manuscript; K. Maundrell for the fission yeast nmt1 promoter; and K. Lundgren and D. Beach for strain SP984. This work was supported by the American Cancer Society (P.B.C.) and the Helen Hay Whitney Foundation (P.R.M.). P.R.M. is an associate and W.G.D. is an investigator of the Howard Hughes Medical Institute.Additional details
- Eprint ID
- 57519
- Resolver ID
- CaltechAUTHORS:20150514-080902056
- American Cancer Society
- Helen Hay Whitney Foundation
- Howard Hughes Medical Institute (HHMI)
- Created
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2015-05-14Created from EPrint's datestamp field
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2021-11-10Created from EPrint's last_modified field