Lack of interferon response in animals to naked siRNAs
Abstract
RNA interference (RNAi) is rapidly becoming the method of choice for the elucidation of gene function and the identification of drug targets. As with other oligonucleotide-based strategies, RNAi is envisioned to ultimately be useful as a human therapeutic. Unlike previous nucleic acid therapeutics, small interfering RNAs have the potential to elicit immune responses via interactions with Toll-like receptor 3 and trigger interferon responses like long, double-stranded RNA and its analogs, such as poly(I:C). Recently, the safety of siRNAs has been questioned because they have been shown to trigger an interferon response in cultured cells. We show here that it is possible to administer naked, synthetic siRNAs to mice and downregulate an endogenous or exogenous target without inducing an interferon response.
Additional Information
© 2004 Macmillan Publishers Limited. Received 17 May; accepted 30 September 2004. The authors would like to thank Jean Lee and Hu Wong for critical blood chemistry and liver panel analyses. We thank Anton McCaffrey and Mark Kay for a donation of the plasmid used in our studies. J.D.H. acknowledges the Whitaker Foundation for a doctoral fellowship. S.H. is supported by an endowment in Molecular Pathology from the Las Madrinas Foundation at CHLA.Attached Files
Supplemental Material - nbt1038-S1.pdf
Supplemental Material - nbt1038-S2.pdf
Supplemental Material - nbt1038-S3.pdf
Supplemental Material - nbt1038-S4.pdf
Supplemental Material - nbt1038-S5.pdf
Supplemental Material - nbt1038-S6.pdf
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Additional details
- Eprint ID
- 57485
- Resolver ID
- CaltechAUTHORS:20150513-095404544
- Whitaker Foundation
- Las Madrinas Foundation
- Created
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2015-05-13Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field