Becoming a new neuron in the adult olfactory bulb
Abstract
New neurons are continually recruited throughout adulthood in certain regions of the adult mammalian brain. How these cells mature and integrate into preexisting functional circuits remains unknown. Here we describe the physiological properties of newborn olfactory bulb interneurons at five different stages of their maturation in adult mice. Patch-clamp recordings were obtained from tangentially and radially migrating young neurons and from neurons in three subsequent maturation stages. Tangentially migrating neurons expressed extrasynaptic GABAA receptors and then AMPA receptors, before NMDA receptors appeared in radially migrating neurons. Spontaneous synaptic activity emerged soon after migration was complete, and spiking activity was the last characteristic to be acquired. This delayed excitability is unique to cells born in the adult and may protect circuits from uncontrolled neurotransmitter release and neural network disruption. Our results show that newly born cells recruited into the olfactory bulb become neurons, and a unique sequence of events leads to their functional integration.
Additional Information
© 2003 Nature Publishing Group. Received 6 February; Accepted 11 March 2003. This work was supported by the Pasteur Institute, the Annette Gruner-Schlumberger Foundation, the CNRS, the Fondation pour la Recherché Médicale, a grant from the French Ministry of Research and Education (ACI Biologie du Développement et Physiologie Intégrative 2000) and by a National Institutes of Health grant HD32116. We thank E. Perret and M.M. Gabellec for technical help with the confocal microscope and immunocytochemistry, respectively. We also thank A. Saghatelyan, M. Davenne and I. Manns for critical reading of the manuscript.Attached Files
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Additional details
- Eprint ID
- 56878
- DOI
- 10.1038/nn1048
- Resolver ID
- CaltechAUTHORS:20150422-114741426
- Pasteur Institute
- Annette Gruner-Schlumberger Foundation
- Centre National de la Recherche Scientifique (CNRS)
- Fondation pour la Recherche Médicale
- ACI Biologie du Développement et Physiologie Intégrative 2000
- HD32116
- NIH
- Created
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2015-04-22Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field