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Published June 1, 2000 | public
Journal Article

In vivo functional cooperation of insulin-like growth factor I and leukemia inhibitory factor studied in mutant mice

Abstract

Leukemia Inhibitory Factor (LIF) and Insulin like growth factor I (IGFI) are multifunctional proteins that regulate cell survival, proliferation or differentiation of cell types of several lineages. They are expressed in mammalian development in lung, muscle, skin, the nervous system and other tissues. Previous results indicate cross-talk between the signalling mediators of L1F (STAT proteins) and IGF-I (1RS proteins). To study a possible cooperation in vivo between LIF and IGF-I, heterocygous mutant mice (kindly provided by C. L. Stewart and A. Efstratiadis respectively) were intercrossed. Mortality before weaning was low for LIF-nult mice (13%) whereas, as previously reported, it was significant for IGF-Iqmll mice (62%). All double-null mutant mice died at birth suggesting a cooperation of LIF and tGF-I in vital functions, as well as sustaining embryonic growth. In E18.5, the deficit in LIF aggravates the lung, muscle, and skin abnormalities present in IGF-I deficient mice. Whereas most of the central nervous system areas appeared grossly normal in single and double mutants, there was a 70% depletion of mitral neurons in the olfactory bulb, depending almost exclusively of IGF-I deficit. Thus, these two growth factors have cooperative and independent actions in different tissues during development.

Additional Information

© 2000 by Academic Press.

Additional details

Created:
August 19, 2023
Modified:
October 20, 2023