Molecular Distinction and Angiogenic Interaction between Embryonic Arteries and Veins Revealed by ephrin-B2 and Its Receptor Eph-B4
Abstract
The vertebrate circulatory system is composed of arteries and veins. The functional and pathological differences between these vessels have been assumed to reflect physiological differences such as oxygenation and blood pressure. Here we show that ephrin-B2, an Eph family transmembrane ligand, marks arterial but not venous endothelial cells from the onset of angiogenesis. Conversely, Eph-B4, a receptor for ephrin-B2, marks veins but not arteries. ephrin-B2 knockout mice display defects in angiogenesis by both arteries and veins in the capillary networks of the head and yolk sac as well as in myocardial trabeculation. These results provide evidence that differences between arteries and veins are in part genetically determined and suggest that reciprocal signaling between these two types of vessels is crucial for morphogenesis of the capillary beds.
Additional Information
© 1998 by Cell Press. Under an Elsevier user license. Received 14 April 1998, Revised 5 May 1998, Available online 29 September 2000. We thank P. Mombaerts for the tau-lacZ plasmid, A. Bradley and R. Behringer for AB-1 ES cells, L. Wang for feeder cell culture, B. Turring for illustrations, the staff of the Caltech Transgenic Facility for mouse care, and lab members for discussion and support. We thank J. Folkman for an insightful review and J. L. Anderson for encouragement. This work was supported by a grant from the American Paralysis Association. D. J. A. is an Investigator of the Howard Hughes Medical Institute.Additional details
- Eprint ID
- 55235
- Resolver ID
- CaltechAUTHORS:20150226-070639170
- American Paralysis Association
- Howard Hughes Medical Institute (HHMI)
- Created
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2015-02-26Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field