Nucleotide sequence of the Methylobacterium extorquens AM1 moxF and moxJ genes involved in methanol oxidation
Abstract
The nucleotide sequence has been determined for two genes involved in methanol oxidation in the facultative methylotroph, Methylobacterium extorquens AM1. The two genes are moxF, encoding the 66-kDa subunit of the methanol dehydrogenase and moxJ, located immediately downstream from moxF, which encodes a 30-kDa protein with unknown function. This information completes the sequence of the 5.86-kb XhoI-SalI fragment containing the moxFJGI region in M. extorquens AM1, and the structure of this gene cluster is presented. Evidence is presented that moxJ is also present in Paracoccus denitrificans. The aa sequence of MoxJ has provided little information concerning its function, but it does appear to contain a signal sequence suggesting a periplasmic location.
Additional Information
© 1990 Elsevier Science Publishers B.V. Received 5 December 1989, Revised 17 February 1990, Accepted 20 February 1990, Available online 19 December 2002. Received by M. Bagdasarian. This work was supported by a grant to M.E.L. from the US Department of Energy (No. DE-FG03-87ER13753). D.N.N. was supported by an NIH Post-doctoral fellowship. C.A. was supported by SERC.Additional details
- Eprint ID
- 55186
- DOI
- 10.1016/0378-1119(90)90457-3
- Resolver ID
- CaltechAUTHORS:20150225-084708010
- DE-FG03-87ER13753
- Department of Energy (DOE)
- NIH Postdoctoral Fellowship
- Science and Engineering Research Council (UK)
- Created
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2015-02-25Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field