Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published December 2014 | Erratum + Published + Supplemental Material
Journal Article Open

MORC1 represses transposable elements in the mouse male germline

Abstract

The Microrchidia (Morc) family of GHKL ATPases are present in a wide variety of prokaryotic and eukaryotic organisms but are of largely unknown function. Genetic screens in Arabidopsis thaliana have identified Morc genes as important repressors of transposons and other DNA-methylated and silent genes. MORC1-deficient mice were previously found to display male-specific germ cell loss and infertility. Here we show that MORC1 is responsible for transposon repression in the male germline in a pattern that is similar to that observed for germ cells deficient for the DNA methyltransferase homologue DNMT3L. Morc1 mutants show highly localized defects in the establishment of DNA methylation at specific classes of transposons, and this is associated with failed transposon silencing at these sites. Our results identify MORC1 as an important new regulator of the epigenetic landscape of male germ cells during the period of global de novo methylation.

Additional Information

© 2014 Macmillan Publishers Limited. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ Received 12 September 2014; Accepted 7 November 2014; Published 12 December 2014. We acknowledge the UCLA BSCRC Flow Cytometry core for flow and FACS assistance, and the UCLA BSCRC High Throughput Sequencing Core. Bryan Cullen provided anti-IAP Gag antibody and Alexander Bortvin provided anti-LINE ORF1p antibody. Jiamu Du and Dinshaw Patel provided recombinant MORC1 coiled-coil domain to raise antibody. W.A.P. is supported by the Jane Coffin Childs Memorial Fund for Medical Research. H.S. is an HHMI Fellow of the Damon Runyon Cancer Research Foundation (DRG-2194-14). W.L. is supported by a grant from the Chinese Scholar Council. This work was supported by grants from the NIH (R01 HD058047 and DP2 OD007371A), a Research Award from the Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research and the Nogradi Fund, the Searle Scholar and the Packard Fellowship Awards, and an ANR grant ("TranspoFertil"). S.E.J. is an investigator of the Howard Hughes Medical Institute.

Attached Files

Published - ncomms6795.pdf

Supplemental Material - ncomms6795-s1.pdf

Supplemental Material - ncomms6795-s2.xlsx

Supplemental Material - ncomms6795-s3.xlsx

Supplemental Material - ncomms6795-s4.xlsx

Supplemental Material - ncomms6795-s5.xlsx

Supplemental Material - ncomms6795-s6.xlsx

Erratum - ncomms8604.pdf

Files

ncomms6795-s1.pdf
Files (6.6 MB)
Name Size Download all
md5:bc77587e6d1a4feb89fb9db00f34aac9
3.6 MB Preview Download
md5:ae64c0b881b23c25f373469f03511efc
46.0 kB Download
md5:855273deca7a52634a137eedd42cf7e2
1.8 MB Preview Download
md5:c00031f11a1a8f5844162988a32abfab
324.9 kB Preview Download
md5:035ff505d36bd4d5ec25f33b57854f26
464.3 kB Download
md5:5016c82d4c28cbe07af86fd419171a3e
279.0 kB Download
md5:ea3f29a035476369f35043814650b03c
54.4 kB Download
md5:6aec548c1389dc241cdd392fb7379a69
47.6 kB Download

Additional details

Created:
August 22, 2023
Modified:
October 23, 2023